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| A | 224229 | 185 | Produce CBFish Status Report | Periodic Status Reports for BPA | The Contractor shall report on the status of milestones and deliverables in Pisces. Reports shall be completed either monthly or quarterly as determined by the BPA COTR. Additionally, when indicating a deliverable milestone as COMPLETE, the contractor shall provide metrics and the final location (latitude and longitude) prior to submitting the report to the BPA COTR. | $1,000 | 0.05% | 10/01/2022 | 05/31/2023 |
| B | 224230 | 165 | Produce Environmental Compliance Documentation | NEPA Work for River Restoration Activities as Needed | This work element describes activities to assemble, gather, acquire, or prepare documents in support of obtaining environmental compliance from BPA (such as filling out a NEPA Checklist, providing maps, drafting a Biological Assessment, obtaining permits, conducting public involvement activities, completing an archaeological survey, etc.) in relation to the nutrient restoration effort at the MT-ID border and tributary vegetation plantings on key Kootenai River tributaries. | $12,000 | 0.59% | 07/01/2022 | 05/31/2023 |
| C | 224231 | 44 | Enhance Nutrients in Water Bodies | Kootenai River Nutrient Restoration Mitigation--Nitrogen and Phosphorus Additions | Continuation of a controlled, large-scale nutrient restoration experiment in the mainstem Kootenai River, downstream of the Montana-Idaho border to restore ecosystem productivity. Liquid phosphorus (P) fertilizer will be added (as 10-34-0) to the Kootenai River, and if needed, liquid nitrogen (N: as 32-0-0). Approximately 20,000 gallons of P will be budgeted for. | $493,349 | 24.09% | 07/01/2022 | 05/31/2023 |
| D | 224232 | 157 | Collect/Generate/Validate Field and Lab Data | Biomonitoring--Water Quality | Monitor key water quality parameters, with an emphasis on macro-nutrients needed for in-river biological growth and development, and heavy metals that may be detrimental to food web health. Monthly water quality samples will be collected at 8 sites within distinct reaches of the Kootenai River ecosystem from Troy, MT to Porthill, ID) during the biologically productive seasons (April to September; June to September for this contract). When possible, water samples will be taken from a boat and will integrate water from several depths and locations along a transect representing near-shore and mid-channel flows. Three samples will be taken at each site. Samples will be sent to IEH Analytical Laboratories, Inc., Seattle, WA within 24 hours for analysis.
Total nitrogen, N-ammonia, nitrite-nitrate, and, total, total dissolved, and soluble-reactive phosphorous will be analyzed to the lowest possible detection limit. Additionally, a few samples (approximately 10) will be taken annually at sites directly above and below the nutrient addition point and will be tested for heavy metals that are sometimes associated with phosphorus fertilizers.
Biomonitoring sites include control sites above nutrient addition; within nutrient addition and known zone of influence; and below nutrient addition zone of influence. Results from the different sites will allow for comparisons of water quality parameters, organisms across trophic levels responses, and fish response/metrics comparisons. | $277,085 | 13.53% | 07/01/2022 | 05/31/2023 |
| E | 224233 | 157 | Collect/Generate/Validate Field and Lab Data | Biomonitoring--Algae | Monitor/Evaluate in-river primary productivity (algae) of the Kootenai River as related to large-scale ecosystem restoration efforts. This work element describes monitoring the chlorophyll concentration and biomass of the algal community. Additionally, algae species composition will be monitored and evaluated.
Monthly quantitative algae scrape samples will be collected at 8 sites within distinct reaches of the Kootenai River ecosystem from Troy, MT to Porthill, ID during the biologically productive seasons (April to September). Samples will be taken from Styrofoam® substrates. Two to four Styrofoam substrate samplers will be placed on the bottom of the river one month prior to the start of the spring growth period. Periphyton cores will be punched from each sampler and preserved according to Standard Methods (APHA 1998). Samples will be sent to the University of Idaho Analytical Sciences Lab for chlorophyll analysis.
Additional algae scrape samples will be taken for the purpose of identifying algal species (diatoms, soft- bodied algae) present within the periphyton community (same sites and times as chlorophyll sampling). Cell counts per unit area of substrate and species composition/ diversity of periphyton will be used to determine taxonomic richness and composition at each site. Differences in taxonomic richness will be compared spatially and temporally within reaches and sites. The taxonomic analysis will be performed by EcoAnalysts, Inc., Moscow, Idaho.
Biomonitoring sites include control sites above nutrient addition; within nutrient addition and known zone of influence; and below nutrient addition zone of influence. Results from the different sites will allow for comparisons of water quality parameters, organisms across trophic levels responses, and fish response/metrics comparisons. | $140,233 | 6.85% | 07/01/2022 | 05/31/2023 |
| F | 224234 | 157 | Collect/Generate/Validate Field and Lab Data | Fine-Scale Algae & Water Quality Monitoring | This WE describes localized and intensive water quality and algae monitoring directly related to the nutrient restoration experiment. Six (6) sites, approximately 4 rkms apart (rkm 247.5-287), will be monitored for nutrients (TP, TDP, SRP, TN, NH4, NO3+NO2, and TOC), algal taxonomy, and chlorophyll biomass. Two sites are upstream of the nutrient addition outflow pipe (controls), one site is at the nutrient addition location, and 3 are located downstream of the nutrient outflow pipe (treatment). This will allow river managers to track the effects of the added nutrients at a fine-scale level. The timing of this monitoring will mirror the time frame of the nutrient additions (a 16 week period from June-Sept.). Three water quality samples testing nutrient concentrations will be taken at each site weekly. Four algae core samples to estimate chlorophyll content will be taken at each site bi-weekly. Algae scrape sample will be taken monthly to monitor algal species composition at each of the 4 sites. AFDM samples (3) and filtered chlorophyll samples will also be sampled every two weeks from the natural substrate.
Only limited sampling occurs at sites KRF 2 and 12, where no chlorophyll or periphyton are taken and only one water sample is collected. These sites were established to satisfy the EPA NPDES permit requirements for nitrate and total organic carbon.
Additional samples may be collected to determine Total Organic Carbon (TOC) sources in and near the nutrient addition zone during the spring runoff period prior to the annual start-up of nutrient additions.
Data sets will be analyzed and reported on for chlorophyll biomass, periphyton taxonomy, and water chemistry parameters. | $130,101 | 6.35% | 07/01/2022 | 05/31/2023 |
| G | 224235 | 157 | Collect/Generate/Validate Field and Lab Data | Biomonitoring--Macroinvertebrates | This work element describes monitoring the benthic invertebrate community abundance, biomass, species composition, and, starting in 2020, organic matter content analyses.
Seasonal macro-invertebrate samples will be collected at 8 biomonitoring sites on the Kootenai River from Troy, MT to Porthill, ID, representing the major reaches of the river. Five macroinvertebrate samples will be taken at each site along a transect, with representation of near shore and mid-channel habitats, when possible. Data sets of benthic macroinvertebrates will be collected with quantitative gear (Slack and Ponar samplers) appropriate to the site and reach. Five macroinvertebrate samples will be taken at each site along a transect, with representation of near shore and mid-channel habitats (if possible) at 8 sites within distinct reaches of the Kootenai River ecosystem from Troy, MT to Porthill, ID during the biologically productive seasons, with one complete set of samples taken per season (i.e. Spring, Summer, and Fall). Invertebrate data sets will be analyzed for abundance, biomass, and taxonomic composition.
EcoAnalyst, Inc. will sort samples from debris and perform taxonomic analysis, biomass determination, and analyses of community attributes (i.e. functional feeding classifications, and relative river health indices). | $141,213 | 6.90% | 07/01/2022 | 05/31/2023 |
| H | 224236 | 157 | Collect/Generate/Validate Field and Lab Data | Fish Species/Community Dynamics & Abundance | This work element describes monitoring fish species/community dynamics and abundance. Fish community monitoring is a collaborate effort between the Idaho Department of Fish and Game, the Kootenai Tribe of Idaho, and, the Montana Department of Fish, Wildlife, and Parks.
The relative abundance of fishes will be assessed at eight sites along the Kootenai River continuum. Six sites are in Idaho, and two sites are in Montana. Since the river is large and an open system, fish population estimates will be assessed as relative abundance (number and biomass per hour). Fish collections will be made by boat-electrofishing and will be performed in near-shore habitats (2-10 ft.) at night. Sampling will be conducted the first two weeks of September each year. A one kilometer stretch of river shoreline will be electro-fished on each side of the Kootenai River at each site (totaling 2 km). Six samples, 333 meters in length, will be taken within the 2km to derive a better estimate (a variance measure is added) of relative abundance and biomass. Two netters will collect all fish without intentional bias towards size or species. All fish will be weighed, measured, and recorded. | $120,213 | 5.87% | 07/01/2022 | 05/31/2023 |
| I | 224237 | 44 | Enhance Nutrients in Water Bodies | Kootenay Lake Nutrient Restoration | This Work Element describes nutrient addition and monitoring on Kootenay Lake, B.C. (part of lower Kootenai River ecosystem). The purpose of the work is to recover lost system productivity created by construction and operation of Libby Dam. The Kootenai River, historically, provided approximately 60 percent of the annual nutrient budget for Kootenay Lake. Nutrient enhancement started in the north Arm of the lake circa 1992 with funds from the British Columbia Government and BC Hydro. Fisheries response to nutrient addition efforts has been positive, with large gains in kokanee and rainbow and bull trout, although cyclic patterns do occur in predator and prey populations from low to high, even with nutrient additions. The populations are currently in mid-cycle after a recent low period.
Since 2003, this project has contributed monies to the British Columbia Government Fisheries department in Nelson, B.C. to add nutrients and monitor the results in the South Arm of Kootenay Lake. This work is aimed to bolster populations of kokanee and other fish species that migrate between Kootenay Lake and the lower Kootenai River near Bonners Ferry, Idaho. In particular, the nutrients are aimed to recover kokanee, white sturgeon, and burbot, species that use both the lower Kootenai River and Kootenay Lake. The response of kokanee and trout in the South Arm has been very positive to date.
Activities performed at Kootenay Lake include: collection and analysis of water chemistry and phytoplankton samples collected (0-20m integrated, and hypolimnetic water chemistry samples), at four Kootenay Lake stations; collection and analysis of discrete water chemistry and phytoplankton samples collected (2, 5, 10, 15 and 20 m) at two Kootenay Lake stations; zooplankton and mysid samples will be collected at four Kootenay Lake stations; primary production samples will be collected and analyzed, as will bacteria and seston samples at two Kootenay Lake stations. South Arm Kootenay Lake kokanee escapement counts in tributaries will be conducted during August/September. Additionally, monies will be used to conduct an annual hydro acoustic survey to determine distribution, abundance and fry size of kokanee.
During this Period, nitrogen fertilizer will be added (as nitrogen; 28-0-0) to the South Arm of Kootenay Lake commencing in mid-July (added on a weekly basis) through August. Based on past year’s averages, approximately 920 tonnes (metric tons) of nitrogen fertilizer will be applied to the South Arm during the application period. | $589,445 | 28.79% | 07/01/2022 | 05/31/2023 |
| J | 224238 | 160 | Create/Manage/Maintain Database | Kootenai River Ecosystem Project Data Base | This WE describes work to maintain and further develop a web-based database to store the large volume of data generated by the Kootenai Tribe's Ecosystem Project responsible for monitoring water quality and the biological trophic levels within the river at 10+ sites in any given year. Information generated from the KTOI's monitoring program will be organized and sent to the database manager to the web-based database during and after the field season by project staff. Data is housed and maintained at Guem, Ltd., Hamilton, MT.
Specific work planned for the Relational Database during this contract period includes:
1. Inclusion and uploading of trophic level and water chemistry data,
2. Maintenance and possible upgrades as needed.
All database activities will be overseen and reviewed by the KTOI project manager and KTOI Fish and Wildlife Department Administrator as needed. | $48,000 | 2.34% | 07/01/2022 | 05/31/2023 |
| K | 224239 | 183 | Produce Journal Article | Assistance with Reporting, Publications, and Public Relations Documents | WE outlines professional assistance needed to meet project goals.
Professional assistance may be used to assist with any of the following: analysis and interpretation of monitoring and evaluation data (as outlined in objectives and tasks), and to help organize, analyze, and summarize any previous years data. This also includes travel and lodging expenses for visiting scientists to and from Bonners Ferry, ID or other meeting locations.
I. Hay, Inc. will:
1. Provide a written summary of the Kootenai River Nutrient Dosing System and N-P consumption for the nutrient addition field season. (14 d).
2. Prepare, contribute to, and edit project research publications on an as needed basis.
II. AquaFish will:
1. Provide a written summary of the Kootenai River water and algae sampling efforts in support of a second nutrient site.
2. Finish a Mountain Whitefish Fish diet summary report. | $60,000 | 2.93% | 07/01/2022 | 05/31/2023 |
| L | 224241 | 119 | Manage and Administer Projects | Development of SOW, LIB, Metric Reporting and yearly Accruals | This Work Element involves the development of a yearly statement of work (SOW), a line-item budget, and, accrual and metric reporting for project 199404900, Kootenai River Resident Fish Assessment.
A total of 3-4 weeks of project manager's time will be needed to complete these tasks.
Overall budget for this WE includes project personnel, indirect, fringe benefit costs and other costs not included in the other WE's for project 1994-049-00. | $5,000 | 0.24% | 07/01/2022 | 05/31/2023 |
| M | 224242 | 132 | Produce Progress (Annual) Report | Submit Progress Report for the period June 1, 2021 to May 31, 2022 Previous Performance period. | Provide written summaries of all research and management activities for project 1994-049-00. The principle investigator for project 1994049000 will submit an annual report of research and management activities in a thorough and timely manner. Reports concerning topics outside the expertise of the project principle investigator will be done on a sub-contract basis, and also provided in a timely manner. | $30,000 | 1.47% | 07/01/2022 | 05/31/2023 |
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